FBXW7 Knockout cell line(SW480)

FBXW7 Knockout cell line(SW480) Order

Catalog#

YKO-H499

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FBXW7 Knockout cell line (SW480)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

FBXW7

Gene ID

55294

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 4 (based on transcript FBXW7-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SW480			STR Info (Cell bank) Cell Line:SW-480
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	12	12		12	12
D7S820	8	8		8	8
D16S539	13	13		13	13
VWA	16	16		16	16
TH01	8	8		8	8
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	13	14		13	14

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FBXW7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SW480 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FBXW7 gene knockout FBXW7 Knockout cell line (SW480) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Human Colon Cancer Cell Line(SW480)
More details Order

Catalog#: YC-C057

Size: 1*10^6

Instruction:

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

FBXW7 Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-H702

YKO-H702(HEK293)
FBXW7 Knockout cell line(5637)
inquiry More details

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-H519

YKO-H519(5637)
FBXW7 Knockout cell line (Hela)
inquiry More details

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-XN88447

YKO-XN88447(Hela)

Related Popular Products

Wild-type Cell Lines

Human Colon Cancer Cell Line(SW480)

Price (USD): -

Catalog#: YC-C057

Size: 1*10^6

Instruction:

inquiry More details

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

FBXW7 Knockout cell line (HEK293)

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-H702

inquiry More details
FBXW7 Knockout cell line(5637)

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-H519

inquiry More details
FBXW7 Knockout cell line (Hela)

Organism: Human

Gene Name: FBXW7

Gene ID: 55294

Size: 1*10^6

Catalog#: YKO-XN88447

inquiry More details

FBXW7 Knockout cell line(SW480)

Ubigene